Introduction to RNA-seq Data Processing and Differential Gene Expression Analysis with Pseudomonas putida

Cabebe, Gabrielle

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Introduction to RNA-seq Data Processing and Differential Gene Expression Analysis with Pseudomonas putida

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Ever since its introduction in 2008, RNA sequencing (RNA-seq) has become an indispensable tool for transcriptome analysis [1]. With the copious amount of RNA-seq data being generated, a new challenge of extracting the most meaning out of this data with as little misinterpretation as possible. This data extraction requires appropriate background and computational skills, as well as the necessary tools to carry this out. More thorough documentation for RNA-seq processing is available for the most studied organisms, such as E. coli and human tissues. However, these resources may be more difficult to locate for lesser known organisms. The goals of this workflow is to break down each step of the RNA-seq data processing using the prokaryotic organism Pseudomonas putida.

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